The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein(paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney(MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
C-terminal partial recombinant chicken paxillin (amino acids 305-559) was used as the immunogen for this Paxillin antibody.
Isotype
IgG1
Predicted Reactivity
Human, mouse, rat, chicken
Reactivity
Human, Mouse, Rat, Chicken
Recombinant
No
Gene Id
5829
Clone No
PXC-10
Format
Ascites
Purification
Ascites
Storage
After reconstitution, the Paxillin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, ICC
Dilution
Western blot: 1-2ug/ml,Immunocytochemistry: Suitable