Rabbit Anti-Human p70 S6K Polyclonal

Mfr.No.	SPC-146, SPC-146F, SPC-146F-A390, SPC-146F-A488, SPC-146F-A594, SPC-146F-BI, SPC-146F-FITC
Immunogen Species	Human
Alternative Names	p70 S6 kinase beta (phospho T229) Antibody, p70-beta Antibody, p70S6Kb Antibody, S6K beta Antibody, S6K2 Antibody, SRK Antibody, STK14B Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus, Cell Junction, Mitochondrion, Mitochondrion Outer Membrane
Certificate Of Analysis	0.25 mg/ml was sufficient for detection of SPC-146 in lysates prepared from mouse muscle and mouse brain.
Clonality	Polyclonal
Description	Rabbit Anti-Human p70 S6K Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Human p70 S6K c-terminal peptide –KLH conjugates, Homo Sapiens (Human)
Immunogen Region	Human p70 S6K c-terminal peptide KLH conjugates
Launch Date	2007/06/01
Net Weight (g)	0.4
Reactivity	Rat, Mouse, Human, Bovine
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Bovine, Human

Swissprot	Q9UBS0
Accession Number	NP_003943.2
Gene Id	6199
Target	p70 S6K
Research Area	Cell Signaling, Post-translational Modifications, Phosphorylation

Buffer	TBS, 50% glycerol, 0.01% sodium azide
Concentration	0.25 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~70kDa.
Storage	-20ºC
Storage Buffer	TBS, 50% glycerol, 0.01% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IP, WB, ICC/IF
Description	WB ; ICC ; IF ; IP ; ELISA
Dilution	WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Gout, I. et al. (1998). J Biol Chem. 273 (46):30061-30064. 2. Val'Ovka, TI. et al. (2000). Ukr Biokhim Zh. 72(3):31-7. 3. Kawasome, H. et al. (1998) Proc. Natl. Acad.Sci. 95 4. Montagne, J. et al. 1999) Science 285 5. Shima, H. et al. (1998) EMBO J 17: 6649-6659. 6. Lee-Fruman, K. et al. (1999) Oncogene 18: 5108- 5114.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906719-1	SPC-146F	Unconjugated	400 ul	$202	Add to Cart
BHA11906719-2	SPC-146F-A390	ATTO 390	400 ul	$256	Add to Cart
BHA11906719-3	SPC-146F-A488	ATTO 488	400 ul	$255	Add to Cart
BHA11906719-4	SPC-146F-A594	ATTO 594	400 ul	$255	Add to Cart
BHA11906719-5	SPC-146F-BI	Biotin	400 ul	$250	Add to Cart
BHA11906719-6	SPC-146F-FITC	FITC	400 ul	$245	Add to Cart

p70 S6K Antibody - SPC-146