Rabbit Anti-Human p53 (pSer315) Polyclonal

Mfr.No.	SPC-1411, SPC-1411D, SPC-1411D-A390, SPC-1411D-A488, SPC-1411D-A594, SPC-1411D-BI, SPC-1411D-FITC
Immunogen Species	Human
Alternative Names	p53 tumor suppressor Antibody, Cellular tumor antigen p53 Antibody, Antigen NY-CO-13 Antibody, Phosphoprotein p53 Antibody, Tumor protein 53 Antibody, BCC7 Antibody, p53 Antibody, TP53 Antibody, P53_HUMAN Antibody, FLJ92943 Antibody, Mutant tumor protein 53 Antibody, Tumor protein p53 Antibody, LFS1 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor suppressor p53 Antibody
Antigen	p53
Categories	Antibodies
Cellular Localization	Endoplasmic Reticulum, Pml Body, Intracellular, Mitochondrion Matrix, Nucleus Pml Body, Nucleus, Cytoplasm, Cytosol, Mitochondrion, Nuclear Chromatin, Nuclear Matrix, Nucleolus, Nucleoplasm, Protein Complex, Replication Fork
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human p53 (pSer315) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic phospho-peptide from human p53 around the phosphorylation site of serine 315 (pSer315)., Homo Sapiens (Human)
Immunogen Region	Synthetic phospho-peptide from human p53 around the phosphorylation site of serine 315 (pSer315).
Launch Date	2018/03/07
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human
Tissue Specificity	Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

Swissprot	P04637
Accession Number	NP_000537.3
Gene Id	7157
Target	p53 (pSer315)
Research Area	Cancer, Cell Cycle, p53 Pathway, Tumor suppressors, Apoptosis, Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair

Buffer	PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
Dilutions	WB (1:250-1:1000); IHC (1:50-1:150); IP (1-2.5 ug per mg lysate); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of p53 protein only when phosphorylated at serine 315 (pSer315). Detects a band at ~43 kDa.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC, IP, WB
Description	WB ; IHC ; IP ; ELISA
Dilution	WB (1:250-1:1000); IHC (1:50-1:150); IP (1-2.5 ug per mg lysate); ELISA (1:5000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11919584-1	SPC-1411D	Unconjugated	100 ul	$331	Add to Cart
BHA11919584-2	SPC-1411D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11919584-3	SPC-1411D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11919584-4	SPC-1411D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11919584-5	SPC-1411D-BI	Biotin	100 ul	$380	Add to Cart
BHA11919584-6	SPC-1411D-FITC	FITC	100 ul	$375	Add to Cart

p53 Antibody (pSer315) - SPC-1411

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