Rabbit Anti-Human p27 Kip1 Polyclonal

Mfr.No.	SPC-1282, SPC-1282D, SPC-1282D-A390, SPC-1282D-A488, SPC-1282D-A594, SPC-1282D-BI, SPC-1282D-FITC
Immunogen Species	Human
Alternative Names	Cdki1b Antibody, CDKN 1B Antibody, CDKN 4 Antibody, Cyclin Dependent Kinase Inhibitor 1B Antibody, Cyclin dependent kinase inhibitor p27 Antibody, Cyclin-dependent kinase inhibitor p27 Kip1 Antibody, KIP 1 Antibody, MEN1B Antibody, MEN4 Antibody, p27 Antibody, p27 Kip1 Antibody
Categories	Antibodies
Cellular Localization	Nucleus, Cytoplasm, Endosome
Certificate Of Analysis	A 1:100 dilution of SPC-1282 was sufficient for detection of p27 Kip1 in human brain tissue using Goat Anti-Rabbit IgG as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human p27 Kip1 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthesized peptide derived from Internal of human p27 Kip1., Homo Sapiens (Human)
Immunogen Region	Synthesized peptide derived from Internal of human p27 Kip1.
Isotype	IgG
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human
Tissue Specificity	Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Dilutions	IHC (1:100), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Scientific Background	The cyclin-dependent kinase inhibitor, p27 Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of total p27 Kip1 protein.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC
Description	IHC ; ELISA
Dilution	IHC (1:100), ELISA (1:40000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23. 2. Polyak, K. et al. (1994) Genes Dev 8, 9-22. 3. Kato, J.Y. et al. (1994) Cell 79, 487-96. 4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11917280-1	SPC-1282D	Unconjugated	100 ul	$286	Add to Cart
BHA11917280-2	SPC-1282D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11917280-3	SPC-1282D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11917280-4	SPC-1282D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11917280-5	SPC-1282D-BI	Biotin	100 ul	$334	Add to Cart
BHA11917280-6	SPC-1282D-FITC	FITC	100 ul	$330	Add to Cart

p27 Kip1 Antibody - SPC-1282