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Home / Primary Antibodies / Monoclonal Antibodies

Nitrotryptophan Antibody, Clone 4F8 - SMC-519

SKU : BHA11905654

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StressMarq Biosciences Inc.

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Mfr.No.	SMC-519, SMC-519D, SMC-519D-A390, SMC-519D-A488, SMC-519D-A594, SMC-519D-ALP, SMC-519D-APC, SMC-519D-BI, SMC-519D-FITC, SMC-519D-HRP, SMC-519D-PCP, SMC-519D-RPE, SMC-519S
Alternative Names	6-Nitrotryptophan Antibody, Nitrotryptophan (NW) Antibody, Nitrotryptophan Antibody, NW Antibody, 6-NO2-Trp Antibody
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-519 was sufficient for detection of 6-Nitrotryptophan in 1 ug of 6-Nitrotyrptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Clonality	Monoclonal
Description	Mouse Anti-6-Nitrotryptophan (NW) Monoclonal IgG2b
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Region	Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
Isotype	IgG2b
Launch Date	2017/03/23
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	Nitrotryptophan
Clone No	4F8
Research Area	Cell Signaling, Post-translational Modifications, Nitration

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	6-nitrotryptophan is a major nitrated product of tryptophan residues in proteins reacted with reactive nitrogen species (RNS) (1). Superoxide (O2-) and nitric oxide (NO) react to form peroxynitrous acid, which subsequently nitrates tryptophan residues, modulating protein functions (2). Tryptophan residues are more likely to be nitrated when the indole ring is exposed to the surface of proteins (1).
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C
Trademark	MOLECULAR SIGNATURE

Applications	ELISA, WB, ICC/IF, IHC
Description	WB ; ICC ; IF ; ELISA
Dilution	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1.Yamakura, F. et al. (2007) Intl Congress Series 1304:22-32. 2. Degendorfer, G. et al. (2015) Free Radic Biol Med. 89:721-733.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11905654-1	SMC-519D	Unconjugated	100 ug	$409		Add to Cart
BHA11905654-2	SMC-519D-A390	ATTO 390	100 ug	$463		Add to Cart
BHA11905654-3	SMC-519D-A488	ATTO 488	100 ug	$462		Add to Cart
BHA11905654-4	SMC-519D-A594	ATTO 594	100 ug	$462		Add to Cart
BHA11905654-5	SMC-519D-ALP	Alkaline Phosphatase	100 ug	$455		Add to Cart
BHA11905654-6	SMC-519D-APC	APC	100 ug	$461		Add to Cart
BHA11905654-7	SMC-519D-BI	Biotin	100 ug	$458		Add to Cart
BHA11905654-8	SMC-519D-FITC	FITC	100 ug	$453		Add to Cart
BHA11905654-9	SMC-519D-HRP	HRP	100 ug	$449		Add to Cart
BHA11905654-10	SMC-519D-PCP	PerCP	100 ug	$461		Add to Cart
BHA11905654-11	SMC-519D-RPE	RPE	100 ug	$459		Add to Cart
BHA11905654-12	SMC-519S	Unconjugated	12 ug	$44		Add to Cart

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