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Home / Antibodies

mtTFA Antibody / Tfam (RQ6444)

SKU : BHA17111079

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NSJ Bioreagents

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Mfr.No.	RQ6444
Clonality	Polyclonal
Description	TFAM (Transcription factor A, mitochondrial), also known as mtTFA, TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al.(1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott(2007) stated that the sequences mapped to chromosomes 7p(TCF6L1) and 11q(MTTF1, or TCF6L3) are pseudogenes. Larsson et al.(1997) mapped the mouse mitochondrial transcription factor A gene(Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Recombinant mouse protein (amino acids M1-D211) was used as the immunogen for the mtTFA antibody.
Isotype	IgG
Predicted Reactivity	Mouse, Rat
Reactivity	Mouse, Rat
Recombinant	No
Subcellular Location	Nuclear, cytoplasmic

Uniprot	P40630

Buffer	Lyophilized from 1X PBS with 2% Trehalose
Format	Purified
Purification	Antigen affinity purified
Storage	After reconstitution, the mtTFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB, Direct ELISA
Dilution	Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml

Product Manual	Data Sheet

No Description Available.

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SKU	Supplier No.	Size	Your price	Quantity
BHA17111079	RQ6444	100 ug	$419		Add to Cart

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