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Home / Primary Antibodies

Mono-Methyl-Histone H3 (Lys9)/H3K9me1 Antibody

SKU : BHA12907763

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	DF6936
Alternative Names	; H3.3A ; HIST1 cluster, H3E ; H3 histone family, member A ; H3.1 ; H3/l ; H3F3 ; H3FF ; H3FJ ; H3FL ; Histone gene cluster 1, H3 histone family, member E ; histone H3.1t ; Histone H3/o ; FLJ92264 ; H 3 ; H3 ; H3 histone family, member B ; H3 histone family, member C ; H3 histone family, member D ; H3 histone family, member F ; H3 histone family, member H ; H3 histone family, member I ; H3 histone family, member J ; H3 histone family, member K ; H3 histone family, member L ; H3 histone family, member T ; H3 histone, family 3A ; H3/A ; H3/b ; H3/c ; H3/d ; h3/f ; H3/h ; H3/i ; H3/j ; H3/k ; H3/t ; H31_HUMAN ; H3F1K ; H3F3A ; H3FA ; H3FB ; H3FC ; H3FD ; H3FH ; H3FI ; H3FK ; HIST1 cluster, H3A ; HIST1 cluster, H3B ; HIST1 cluster, H3C ; HIST1 cluster, H3D ; HIST1 cluster, H3F ; HIST1 cluster, H3G ; HIST1 cluster, H3H ; HIST1 cluster, H3I ; HIST1 cluster, H3J ; HIST1H3A ; HIST1H3B ; HIST1H3C ; HIST1H3D ; HIST1H3E ; HIST1H3F ; HIST1H3G ; HIST1H3H ; HIST1H3I ; HIST1H3J ; HIST3H3 ; Histone 1, H3a ; Histone 1, H3b ; Histone 1, H3c ; Histone 1, H3d ; Histone 1, H3e ; Histone 1, H3f ; Histone 1, H3g ; Histone 1, H3h ; Histone 1, H3i ; Histone 3, H3 ; histone cluster 1 H3 family member a ; histone cluster 1 H3 family member b ; histone cluster 1 H3 family member c ; histone cluster 1 H3 family member d ; histone cluster 1 H3 family member e ; histone cluster 1 H3 family member f ; histone cluster 1 H3 family member g ; histone cluster 1 H3 family member h ; histone cluster 1 H3 family member i ; histone cluster 1 H3 family member j ; Histone cluster 1, H3a ; Histone cluster 1, H3b ; Histone cluster 1, H3c ; Histone cluster 1, H3d ; Histone cluster 1, H3e ; Histone cluster 1, H3f ; Histone cluster 1, H3g ; Histone cluster 1, H3i ; Histone cluster 1, H3j ; Histone gene cluster 1, H3 histone family, member A ; Histone gene cluster 1, H3 histone family, member B ; Histone gene cluster 1, H3 histone family, member C ; Histone gene cluster 1, H3 histone family, member D ; Histone gene cluster 1, H3 histone family, member F ; Histone gene cluster 1, H3 histone family, member G ; Histone gene cluster 1, H3 histone family, member H ; Histone gene cluster 1, H3 histone family, member I ; Histone gene cluster 1, H3 histone family, member J ; Histone gene cluster 1, H3A ; Histone gene cluster 1, H3B ; Histone gene cluster 1, H3C ; Histone gene cluster 1, H3D ; Histone gene cluster 1, H3E ; Histone gene cluster 1, H3F ; Histone gene cluster 1, H3G ; Histone gene cluster 1, H3H ; Histone gene cluster 1, H3I ; Histone gene cluster 1, H3J ; Histone H 3 ; Histone H3.1 ; Histone H3.2 ; Histone H3.3 ; Histone H3/a ; Histone H3/b ; Histone H3/c ; Histone H3/d ; Histone H3/f ; Histone H3/h ; Histone H3/i ; Histone H3/j ; Histone H3/k ; Histone H3/l ; Histone H3/m ;
Categories	Primary Antibodies
Clonality	polyclonal
Description	Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3.
Molecular Weight	Observed Mol.Wt.: 15kDa. Predicted Mol.Wt.: 16kDa.
Reactivity	Human, Mouse
Specificity	Histone H3K9me1 Antibody detects endogenous levels of total Histone H3K9me1.
Subcellular Location	Nucleus. Chromosome.

Uniprot

Q16695

Gene Id

8290

Sequence

10	20	30	40	50
MARTKQTARK	STGGKAPRKQ	LATKVARKSA	PATGGVKKPH	RYRPGTVALR
60	70	80	90	100
EIRRYQKSTE	LLIRKLPFQR	LMREIAQDFK	TDLRFQSSAV	MALQEACESY
110	120	130
LVGLFEDTNL	CVIHAKRVTI	MPKDIQLARR	IRGERA

Concentration	1mg/ml
Format	Liquid
Purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IF/ICC, ELISA, ChIP, IHC, IP, WB
Dilution	WB1:500-1:2000 IHC1:50-1:200 IF1:50-1:200 IP1:50-1:200 CHIP1:50-1:200, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12907763	DF6936	100ul	$280		Add to Cart
BHA12907763	DF6936	200ul	$350		Add to Cart

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