Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80-90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN�2, and to undergo ubiquitination by MARCH5. MFN�1 has two key domains. One is a coiled�coil region that mediates MFN�1: MFN�1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
Amino acids DQLLEFVTEGSHFVEATYKNPELDRIA from the human protein were used as the immunogen for the Mitofusin 1 antibody.
Isotype
IgG2b
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Subcellular Location
Cytoplasmic (mitochondria)
Uniprot
Q8IWA4
Clone No
3H3
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Purified
Purification
Affinity purified
Storage
After reconstitution, the Mitofusin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IF
Dilution
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml