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Home / Primary Antibodies / IHC Antibodies

MDM2 Antibody

SKU : BHA10559757

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA891046
Alternative Names	Double minute 2 protein; double minute 2; human homolog of; E3 ubiquitin-protein ligase Mdm2; Hdm2
Categories	Primary Antibodies
Clonality	polyclonal
Description	E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation.Jer-Yen Yang, Mol. Cell. Biol., Oct 2006; 26: 7269 - 7282. Lubing Gu, Blood, May 2002; 99: 3367 - 3375. Hongyan Zhu, J. Biol. Chem., Dec 2003; 278: 49286 - 49292. Tatiana Dilla, Endocrinology, Jan 2000; 141: 420.
Host	Rabbit
Immunogen	Synthesized peptide derived from Human MDM2.
Involvement In Disease	Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
Raised In	Rabbit
Reactivity	Human, Rat
Regulatory	RUO
Relevance	E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Jer-Yen Yang, Mol. Cell. Biol., Oct 2006; 26: 7269 - 7282. Lubing Gu, Blood, May 2002; 99: 3367 - 3375. Hongyan Zhu, J. Biol. Chem., Dec 2003; 278: 49286 - 49292. Tatiana Dilla, Endocrinology, Jan 2000; 141: 420.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total MDM2 protein.
Subcellular Location	Nucleus, nucleoplasm, Cytoplasm, Nucleus, nucleolus

Uniprot	Q00987
Database Links	HGNC:6973 OMIM:164785 KEGG:hsa:4193 STRING:9606.ENSP00000417281 UniGene:Hs.484551
Function	E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation
Pathway	p53 signaling pathway PI3K-Akt signaling pathway Cell cycle Cellular senescence Ubiquitin mediated proteolysis FoxO signaling pathway Endocytosis
Protein Families	MDM2/MDM4 family
Tissue Specificity	Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IHC, IF

Description

ELISA ; WB ; IHC ; IF, WB ; ELISA ; IHC ; IF

Dilution

WB	1:500-1:3000
IHC	1:50-1:100
IF	1:100-1:500

Tested Application

ELISA,WB,IHC,IF;WB:1:500-1:3000,IHC:1:50-1:100,IF:1:100-1:500

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559757	CSB-PA891046	100ul	$297		Add to Cart

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