This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Full-length recombinant human IGF1 protein was used as the immunogen for the IGF-1 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse, Rat
Note
Optimal dilution of the IGF-1 antibody should be determined by the researcher.
Uniprot
P05019
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the IGF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
Neutralization, FACS, IF
Description
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-4ug/ml,In-vitro neutralization of biological activity of IGF-1 (order BSA/sodium azide-free format)