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Home / Primary Antibodies / Monoclonal Antibodies

Hsp90 complex Antibody, Clone 8D3 - SMC-109

BHA11900121
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[email protected]
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Swissprot P08238
Accession Number NP_031381.2
Gene Id 3326
Target HSP90
Clone No 8D3
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tumor Biomarkers, Protein Trafficking
Buffer PBS, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions ICC/IF (1:100), IP (1:1000); optimal dilutions for assays should be determined by the user.
Purification PEG Purified
Scientific Background HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7).
Shipping Temperature Blue Ice or 4°C
Specificity Detects 90kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2alpha (eIF-2alpha) kinase (HRI).
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IP, ICC/IF
Description IP ; ICC ; IF
Dilution ICC/IF (1:100), IP (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577.

2. Pearl H, et al. (2001) Adv Protein Chem 59:157-186.

3. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.

4. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.

5. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.

6. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.

7. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.

8. Perdew, G. H. (1988) JBC 263 (27): 13802-13805

9. Dalman, F. C. et al.(1989) JBC 264(33): 19815-19821

10. Uma, S. et al. (1997) JBC 272(17): 11648-11656.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900121-1 SMC-109B Unconjugated 200 ug $446
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BHA11900121-2 SMC-109A Unconjugated 50 ug $202
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BHA11900121-3 SMC-109B-A390 ATTO 390 200 ug $548
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BHA11900121-4 SMC-109B-A488 ATTO 488 200 ug $545
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BHA11900121-5 SMC-109B-A594 ATTO 594 200 ug $545
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BHA11900121-6 SMC-109B-ALP Alkaline Phosphatase 200 ug $531
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BHA11900121-7 SMC-109B-APC APC 200 ug $543
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BHA11900121-8 SMC-109B-BI Biotin 200 ug $535
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BHA11900121-9 SMC-109B-FITC FITC 200 ug $526
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BHA11900121-10 SMC-109B-HRP HRP 200 ug $519
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BHA11900121-11 SMC-109B-PCP PerCP 200 ug $543
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BHA11900121-12 SMC-109B-RPE RPE 200 ug $537
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BHA11900121-13 SMC-109S Unconjugated 12 ug $44
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