Mouse Anti-Chicken HSP70/HSC70 Monoclonal IgG2a

Mfr.No.	SMC-106, SMC-106B, SMC-106A, SMC-106B-A390, SMC-106B-A488, SMC-106B-A594, SMC-106B-ALP, SMC-106B-APC, SMC-106B-BI, SMC-106B-FITC, SMC-106B-HRP, SMC-106B-PCP, SMC-106B-RPE, SMC-106S
Immunogen Species	Chicken
Alternative Names	HSC54 Antibody, HSC70 Antibody, HSC71 Antibody, HSP70 1 Antibody, HSP701/HSP70 2 Antibody, HSP70.1 Antibody, HSP71 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA10 Antibody, HSPA1A Antibody, HSPA1B Antibody, LAP1 Antibody, NIP71 Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	1 ug/ml of SMC-106 was sufficient for detection of HSP70 and HSC70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Chicken HSP70/HSC70 Monoclonal IgG2a
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Chicken HSP70/HSP90 complex, Gallus Gallus (Chicken)
Immunogen Region	Chicken HSP70/HSP90 complex
Isotype	IgG2a
Launch Date	2007/06/01
Net Weight (g)	0.2
Reactivity	Beluga, Guinea Pig, African clawed frog, Fruit Fly, Dog, Pig, Rat, Fish, Sheep, Yeast, Mouse, Human, Bovine, Rabbit, Hamster, Chicken
Recombinant	FALSE
Regulatory	RUO
Species	Gallus Gallus (Chicken)
Species Reactivity Medium	Mouse, Rat, Bovine, Sheep, Dog, Beluga, Fish, Guinea Pig, Pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast, Human

Swissprot	P08106
Accession Number	NP_001006686.1
Gene Id	423504
Target	HSP70/HSC70
Clone No	BB70
Research Area	Cell Signaling, Chaperone Proteins, Cancer, Tumor Biomarkers, Heat Shock, Protein Trafficking

Buffer	PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~72 (HSP) and ~73kDa (HSC).
Storage	-20ºC
Storage Buffer	PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, IP, WB, ICC/IF
Description	WB ; ICC ; IF ; IHC ; IP
Dilution	WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Zho J. (1998) Cell 94 : 471-480. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith D.F., et al, (1993) Mol. Cell. Biol. 13(2): 869-876. 8. Prapapanich V., et al. (1996) Mol. Cell. Biol. 16(11):6200-6207. 9. Fernandez-Funez et al., (2000) Nature 408(6808): 101-106.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11900061-1	SMC-106B	Unconjugated	200 ug	$345	Add to Cart
BHA11900061-2	SMC-106A	Unconjugated	50 ug	$161	Add to Cart
BHA11900061-3	SMC-106B-A390	ATTO 390	200 ug	$448	Add to Cart
BHA11900061-4	SMC-106B-A488	ATTO 488	200 ug	$444	Add to Cart
BHA11900061-5	SMC-106B-A594	ATTO 594	200 ug	$444	Add to Cart
BHA11900061-6	SMC-106B-ALP	Alkaline Phosphatase	200 ug	$432	Add to Cart
BHA11900061-7	SMC-106B-APC	APC	200 ug	$443	Add to Cart
BHA11900061-8	SMC-106B-BI	Biotin	200 ug	$435	Add to Cart
BHA11900061-9	SMC-106B-FITC	FITC	200 ug	$427	Add to Cart
BHA11900061-10	SMC-106B-HRP	HRP	200 ug	$419	Add to Cart
BHA11900061-11	SMC-106B-PCP	PerCP	200 ug	$443	Add to Cart
BHA11900061-12	SMC-106B-RPE	RPE	200 ug	$437	Add to Cart
BHA11900061-13	SMC-106S	Unconjugated	12 ug	$44	Add to Cart

HSP70/HSC70 Antibody, Clone BB70 - SMC-106

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