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Home / Primary Antibodies / Polyclonal Antibodies

HSC70 (HSP73) Antibody - SPC-102

BHA11906244
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[email protected]
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Swissprot P11142
Accession Number NP_006588.1
Gene Id 3312
Target HSC70
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking
Buffer Rabbit Antiserum
Dilutions WB (1:2000), IHC (1:100), ICC/IF (1:250); optimal dilutions for assays should be determined by the user.
Purification Rabbit Antiserum
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
When cells are subjected to metabolic stress (e.g. heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
Shipping Temperature Blue Ice or 4°C
Specificity Detects 73kDa. Does not cross-react with HSP70.
Storage 4ºC
Storage Buffer Rabbit Antiserum *Storage buffer may change when conjugated
Storage Temperature 4°C
Applications Bl, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; Bl
Dilution WB (1:2000), IHC (1:100), ICC/IF (1:250); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Brown C.L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112.

2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17.

3. Rothman J. (1989), Cell 59: 591-601.

4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.

5. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294.

6. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906244-1 SPC-102D Unconjugated 100 ul $296
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BHA11906244-2 SPC-102C Unconjugated 25 ul $138
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BHA11906244-3 SPC-102D-A390 ATTO 390 100 ul $350
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BHA11906244-4 SPC-102D-A488 ATTO 488 100 ul $349
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BHA11906244-5 SPC-102D-A594 ATTO 594 100 ul $349
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BHA11906244-6 SPC-102D-ALP Alkaline Phosphatase 100 ul $342
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BHA11906244-7 SPC-102D-APC APC 100 ul $348
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BHA11906244-8 SPC-102D-BI Biotin 100 ul $344
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BHA11906244-9 SPC-102D-FITC FITC 100 ul $340
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BHA11906244-10 SPC-102D-HRP HRP 100 ul $336
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BHA11906244-11 SPC-102D-PCP PerCP 100 ul $348
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BHA11906244-12 SPC-102D-RPE RPE 100 ul $345
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BHA11906244-13 SPC-102S Unconjugated 12 ul $44
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