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Home / Primary Antibodies

HPV-16 L1 Antibody (V3062)

SKU : BHA17113269

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NSJ Bioreagents

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Mfr.No.	V3062, V3062-20UG, V3062-100UG
Clonality	Monoclonal
Description	Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. (UniProt)<p>The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody. The epitope has been localized to AA 204-210. (Ref 1)
Isotype	IgG2a,kappa
Predicted Reactivity	Type 16 of Human Papilloma Virus (HPV-16)
Reactivity	Type 16 of Human Papilloma Virus (HPV-16)
Recombinant	No
Subcellular Location	Nuclear

Uniprot	P03101
Clone No	CamVir-1

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	IF, IHC-P
Dilution	Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17113269-1	V3062-20UG	20ug	$219		Add to Cart
BHA17113269-2	V3062-100UG	100ug	$499		Add to Cart

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