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Home / Primary Antibodies

HLA-DP + HLA-DQ + HLA-DR Antibody (MHC II) (V3374)

SKU : BHA17113866

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NSJ Bioreagents

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Mfr.No.	V3374, V3374-20UG, V3374-100UG
Antigen	HLA-DP + HLA-DQ + HLA-DR
Clonality	Monoclonal
Description	This mAb reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some auto-immune diseases are associated with certain class II alleles.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
Isotype	IgG1,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Cytoplasmic and cell surface

Uniprot	P04440, P01908, P01909, P01920, P01903
Clone No	CR3/43

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the HLA-DP/DQ/DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	ELISA, FACS, IF, WB, IHC-P
Dilution	ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17113866-1	V3374-20UG	20ug	$219		Add to Cart
BHA17113866-2	V3374-100UG	100ug	$499		Add to Cart

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