Mouse Anti-Human Histone H3 (tri Methyl Lys36) Monoclonal IgG1

Mfr.No.	SMC-251, SMC-251D, SMC-251D-A390, SMC-251D-A488, SMC-251D-A594, SMC-251D-BI, SMC-251D-FITC
Immunogen Species	Human
Alternative Names	H3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
Categories	Antibodies
Cellular Localization	Nucleus, Chromosome
Certificate Of Analysis	A 1:5000 dilution of SMC-251 was sufficient for detection of Histone H3 in 10 ug of Human HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human Histone H3 (tri Methyl Lys36) Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Prepared by immunizing synthetic peptide coupled to KLH., Homo Sapiens (Human)
Immunogen Region	Prepared by immunizing synthetic peptide coupled to KLH.
Isotype	IgG1
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	P68431/Q71DI3/P84243
Target	Histone H3
Clone No	Q12
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, H3, H3 Acetylation, Histones

Buffer	PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:5000); optimal dilutions for assays should be determined by the user.
Scientific Background	Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous Histone H3(tri methyl K36) protein.
Storage	-20ºC
Storage Buffer	PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB
Description	WB
Dilution	WB (1:5000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Wu R., et al. (1981) Cell. 27:321. 2. Strahl B., et al. (2000) Nature. 403:41. 3. Bauer U., et al. (2002) EMBO Rep. 3:39. 4. Frank D., et al. (2003) Gene. 312:135.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11902093-1	SMC-251D	Unconjugated	100 ul	$286	Add to Cart
BHA11902093-2	SMC-251D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11902093-3	SMC-251D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11902093-4	SMC-251D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11902093-5	SMC-251D-BI	Biotin	100 ul	$334	Add to Cart
BHA11902093-6	SMC-251D-FITC	FITC	100 ul	$330	Add to Cart

Histone H3 Antibody (tri Methyl Lys36), Clone Q12 - SMC-251