Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal

Mfr.No.	SPC-1251, SPC-1251D, SPC-1251D-A390, SPC-1251D-A488, SPC-1251D-A594, SPC-1251D-BI, SPC-1251D-FITC
Immunogen Species	Human
Alternative Names	H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody
Categories	Antibodies
Cellular Localization	Nucleus, Chromosome
Certificate Of Analysis	A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 ug of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E)., Homo Sapiens (Human)
Immunogen Region	Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Isotype	IgG
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	P16104
Accession Number	NP_002096.1
Gene Id	3014
Target	Histone H2A.X (pSer139)
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair, Histones, DNA Damage Recognition

Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	H2AX (H2A histone family, member X) is one of several genes coding for histone H2A. H2AX becomes phosphorylated on serine 139 as a reaction on DNA Double-strand breaks (DSB). The kinases of the PI3-family (Ataxia telangiectasia mutated, ATR and DNA-PKcs) are responsible for this phosphorylation, especially ATM. The role of the phosphorylated form potentially allows for space for the recruitment of proteins necessary during repair of DSBs. In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals. H2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Kuo L.J., and Yang L.X. (2008) In Vivo. 22(3): 305-309. 2. Mukherjee B., et al. (2006) DNA Repair (Amst). 5: 575-90. 3. Solier S., et al. (2009) Mol Cell Biol. 29: 68-82. 4. Lu C., et al. (2006) Mol Cell. 23: 121-32. 5. Lu C., et al. (2008) FEBS Lett. 582: 2703-8.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11916740-1	SPC-1251D	Unconjugated	100 ul	$286	Add to Cart
BHA11916740-2	SPC-1251D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11916740-3	SPC-1251D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11916740-4	SPC-1251D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11916740-5	SPC-1251D-BI	Biotin	100 ul	$334	Add to Cart
BHA11916740-6	SPC-1251D-FITC	FITC	100 ul	$330	Add to Cart

Histone H2A.X Antibody (pSer139) - SPC-1251