Mouse Anti-Yeast HDEL Monoclonal IgG2b

Mfr.No.	SMC-175, SMC-175D, SMC-175C, SMC-175D-A390, SMC-175D-A488, SMC-175D-A594, SMC-175D-ALP, SMC-175D-APC, SMC-175D-BI, SMC-175D-FITC, SMC-175D-HRP, SMC-175D-PCP, SMC-175D-RPE, SMC-175S
Immunogen Species	Yeast
Alternative Names	H-D-E-L (his-asp-glu-leu) Antibody, endoplasmic reticulum Antibody, luminal ER protein retention Antibody, KDELR1 Antibody, Endoplasmic reticulum retention signal Antibody
Antigen	HDEL
Categories	Antibodies
Cellular Localization	Endoplasmic Reticulum
Certificate Of Analysis	1 ug/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 ug of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-Yeast HDEL Monoclonal IgG2b
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip, Saccharomyces Cerivisiae (Yeast)
Immunogen Region	Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Isotype	IgG2b
Launch Date	2010/04/01
Net Weight (g)	0.1
Reactivity	Fruit Fly, Barnyard Grass, Beet, Cotton, Mung Bean, Grass, Wheat, Yeast, Plant
Recombinant	FALSE
Regulatory	RUO
Species	Saccharomyces Cerivisiae (Yeast)
Species Reactivity Medium	Drosophila, Yeast, Plant

Target	HDEL
Clone No	2E7
Research Area	Cancer, Heat Shock, Cell Signaling, Protein Trafficking

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~78kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11901155-1	SMC-175D	Unconjugated	100 ug	$341	Add to Cart
BHA11901155-2	SMC-175C	Unconjugated	25 ug	$179	Add to Cart
BHA11901155-3	SMC-175D-A390	ATTO 390	100 ug	$395	Add to Cart
BHA11901155-4	SMC-175D-A488	ATTO 488	100 ug	$394	Add to Cart
BHA11901155-5	SMC-175D-A594	ATTO 594	100 ug	$394	Add to Cart
BHA11901155-6	SMC-175D-ALP	Alkaline Phosphatase	100 ug	$387	Add to Cart
BHA11901155-7	SMC-175D-APC	APC	100 ug	$393	Add to Cart
BHA11901155-8	SMC-175D-BI	Biotin	100 ug	$389	Add to Cart
BHA11901155-9	SMC-175D-FITC	FITC	100 ug	$385	Add to Cart
BHA11901155-10	SMC-175D-HRP	HRP	100 ug	$381	Add to Cart
BHA11901155-11	SMC-175D-PCP	PerCP	100 ug	$393	Add to Cart
BHA11901155-12	SMC-175D-RPE	RPE	100 ug	$391	Add to Cart
BHA11901155-13	SMC-175S	Unconjugated	12 ug	$44	Add to Cart

HDEL Antibody, Clone 2E7 - SMC-175