GSDMD Antibody

Mfr.No.	AF4012
Clonality	Polyclonal
Host	Rabbit
Immunogen	A synthesized peptide derived from human GSDMD., A synthesized peptide derived from human GSDMD.
Isotype	IgG
Predicted Reactivity	Pig,Horse
Reactivity	Human, Mouse, Rat

Rgd	AB_2846776
Uniprot	P57764
Gene Id	79792
Gene Name	GSDMD

Storage	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	WB, IHC, IF/ICC
Dilution	WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200

Product Manual	Data Sheet

Western blot analysis of extracts from B16F10 cells, using GSDMD Antibody. The lane on the left was treated with blocking peptide.Western blot analysis of extracts from B16F10 cells, using GSDMD Antibody.

Western blot analysis of extracts from various samples, using GSDMD Antibody. Lane 1: C6(TPA + LPS treatment?lysates treated with blocking peptide; Lane 2: C6(TPA treatment? lysates; Lane 3: HepG2(TPA treatment? lysates; Lane 4: C6(TPA and LPS treatment? lysates; Lane 5:HepG2(TPA and LPS treatment? lysates;Western blot analysis of extracts from various samples, using GSDMD Antibody.

AF4012 staining Hela(LPS treated 4h) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF4012 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).AF4012 staining Hela(LPS treated 4h) by IF/ICC.

FIGURE 3?Overexpression of miR-302c-3p reverses ox-LDL-induced pyroptosis in HUVECs. HUVECs were transfected with the NC or miR-302c-3p mimic and then treated with 100 µg/mL ox-LDL. A, qRT-PCR analysis of NLRP3, caspase-1 and IL-1? expression. B and C, Western blot analysis and quantification of NLRP3, GSDMD, casp1 p20 and IL-1? expression. D, Lactate dehydrogenase (LDH) release assay detecting LDH activity in the cell supernatant. E, Representative fluorescence micrographs of cells stained with propidium iodide (PI, red) and Hoechst 33 342 (blue). F, Quantification of the percentage of PI-positive cells. PI-positive cells are reduced in the miR-302c-3p mimic +ox-LDL group compared to those in the NC + ox-LDL group. Scale bars: 50 ?m. *P<.05; **P<.01; ***P<.001. Error bars indicate the mean ±SEM of at least triplicate independent experimentsFIGURE 3?Overexpression of miR-302c-3p reverses ox-LDL-induced pyroptosis in HUVECs.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12913718	AF4012	100ul	$280		Add to Cart
BHA12913718	AF4012	200ul	$350		Add to Cart

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