Mouse Anti-Human GABA A Receptor Alpha5 Monoclonal IgG1

Mfr.No.	SMC-494, SMC-494D, SMC-494D-A390, SMC-494D-A488, SMC-494D-A594, SMC-494D-ALP, SMC-494D-APC, SMC-494D-BI, SMC-494D-FITC, SMC-494D-HRP, SMC-494D-PCP, SMC-494D-RPE, SMC-494S
Immunogen Species	Human
Alternative Names	Gamma-aminobutyric acid receptor subunit alpha-5 Antibody, GABA(A) Receptor subunit Alpha-5 Antibody, Gabra5 Antibody
Categories	Antibodies
Cellular Localization	Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane
Certificate Of Analysis	A 1:100 dilution of SMC-494 was sufficient for detection of GABA-A R, Alpha5 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human GABA A Receptor Alpha5 Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5., Homo Sapiens (Human)
Immunogen Region	Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
Isotype	IgG1
Launch Date	2015/03/10
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	P31644
Accession Number	NP_000801.1
Gene Id	2558
Target	GABA A Receptor
Clone No	S415-24
Research Area	Ion channels, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors, Neurodegeneration, Alzheimer's Disease, Neuroscience

Buffer	PBS pH7.4, 50% glycerol, 0.1% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Hengen K.B., et al. (2012) PLoS One. 7(1): e30608.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11905255-1	SMC-494D	Unconjugated	100 ug	$330	Add to Cart
BHA11905255-2	SMC-494D-A390	ATTO 390	100 ug	$384	Add to Cart
BHA11905255-3	SMC-494D-A488	ATTO 488	100 ug	$383	Add to Cart
BHA11905255-4	SMC-494D-A594	ATTO 594	100 ug	$383	Add to Cart
BHA11905255-5	SMC-494D-ALP	Alkaline Phosphatase	100 ug	$376	Add to Cart
BHA11905255-6	SMC-494D-APC	APC	100 ug	$382	Add to Cart
BHA11905255-7	SMC-494D-BI	Biotin	100 ug	$378	Add to Cart
BHA11905255-8	SMC-494D-FITC	FITC	100 ug	$374	Add to Cart
BHA11905255-9	SMC-494D-HRP	HRP	100 ug	$370	Add to Cart
BHA11905255-10	SMC-494D-PCP	PerCP	100 ug	$382	Add to Cart
BHA11905255-11	SMC-494D-RPE	RPE	100 ug	$380	Add to Cart
BHA11905255-12	SMC-494S	Unconjugated	12 ug	$44	Add to Cart

GABA A Receptor Alpha 5 Antibody, Clone S415-24 - SMC-494