Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A human recombinant partial protein was used as the immunogen for the EPO antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Titering of the EPO antibody may be required for optimal performance.1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Uniprot
P01588
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the EPO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, ELISA, IHC-P
Description
ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT