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Home / Primary Antibodies / Monoclonal Antibodies

ERp57 (GRP58) Antibody, Clone Map.ERp57 (GRP58) - SMC-168

BHA11901056
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+1 866.986.9598
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[email protected]
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Swissprot P30101
Accession Number NP_005304.3
Gene Id 2923
Target ERp57
Clone No Map.ERp57
Research Area Cell Signaling, ER Proteins, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~57kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP
Dilution WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.

2. Williams D.B. (2005) J Cell Sci. 119: 615-623

3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889.

4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582.

5. Oliver J.D., et al. (1997) Science 275: 86-88.

6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226.

7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.

8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305.

9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11901056-1 SMC-168B Unconjugated 200 ug $345
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BHA11901056-2 SMC-168A Unconjugated 50 ug $167
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BHA11901056-3 SMC-168B-A390 ATTO 390 200 ug $448
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BHA11901056-4 SMC-168B-A488 ATTO 488 200 ug $444
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BHA11901056-5 SMC-168B-A594 ATTO 594 200 ug $444
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BHA11901056-6 SMC-168B-ALP Alkaline Phosphatase 200 ug $432
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BHA11901056-7 SMC-168B-APC APC 200 ug $443
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BHA11901056-8 SMC-168B-BI Biotin 200 ug $435
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BHA11901056-9 SMC-168B-FITC FITC 200 ug $427
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BHA11901056-10 SMC-168B-HRP HRP 200 ug $419
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BHA11901056-11 SMC-168B-PCP PerCP 200 ug $443
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BHA11901056-12 SMC-168B-RPE RPE 200 ug $437
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BHA11901056-13 SMC-168S Unconjugated 12 ug $44
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