Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.Ivey-Hoyle M., Mol. Cell. Biol. 13:7802-7812(1993).Gregory S.G., Nature 441:315-321(2006).Novy M., Mol. Biol. Cell 16:2181-2190(2005).
Host
Rabbit
Immunogen
Synthesized peptide derived from internal of Human E2F2.
Raised In
Rabbit
Reactivity
Human, Mouse
Regulatory
RUO
Relevance
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
Ivey-Hoyle M., Mol. Cell. Biol. 13:7802-7812(1993). Gregory S.G., Nature 441:315-321(2006). Novy M., Mol. Biol. Cell 16:2181-2190(2005).
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of total E2F2 protein.
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
Pathway
Cell cycle Cellular senescence
Protein Families
E2F/DP family
Tissue Specificity
Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.