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Home / Primary Antibodies / Monoclonal Antibodies

DNMT1 Antibody, Clone 11H8 - SMC-231

BHA11901866
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+1 866.986.9598
Email
[email protected]
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Swissprot P26358
Accession Number NP_001370
Gene Id 1786
Target DNMT1
Clone No 11H8
Research Area Cell Signaling, Post-translational Modifications, Organelle Markers
Buffer PBS, 0.05% BSA, 0.05% sodium azide, 50% glycerol
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~180kDa. It will cross-react with mouse DNMT1.
Storage -20ºC
Storage Buffer PBS, 0.05% BSA, 0.05% sodium azide, 50% glycerol *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications WB, ICC/IF
Description WB ; ICC ; IF
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983.

2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291.

3. Xie S., et al. (1999) Gene 236: 87-95.

4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257.

5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382.

6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11901866-1 SMC-231D Unconjugated 100 ug $320
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BHA11901866-2 SMC-231D-A390 ATTO 390 100 ug $374
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BHA11901866-3 SMC-231D-A488 ATTO 488 100 ug $373
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BHA11901866-4 SMC-231D-A594 ATTO 594 100 ug $373
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BHA11901866-5 SMC-231D-ALP Alkaline Phosphatase 100 ug $366
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BHA11901866-6 SMC-231D-APC APC 100 ug $372
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BHA11901866-7 SMC-231D-BI Biotin 100 ug $369
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BHA11901866-8 SMC-231D-FITC FITC 100 ug $363
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BHA11901866-9 SMC-231D-HRP HRP 100 ug $359
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BHA11901866-10 SMC-231D-PCP PerCP 100 ug $372
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BHA11901866-11 SMC-231D-RPE RPE 100 ug $370
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BHA11901866-12 SMC-231S Unconjugated 12 ug $44
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