JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.
Loding Loading ....
Home / Primary Antibodies / Monoclonal Antibodies

DNA/RNA Damage Antibody, Clone 15A3 - SMC-155

BHA11900800
Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Request More Infos
Order Now

Credit card payments now incur a 3% fee.

Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Ask Merchant
Request More Infos
Sample Request
Target DNA Damage
Clone No 15A3
Research Area Cancer, Oxidative Stress, Cell Signaling, Post-translational Modifications, Oxidation, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair
Buffer PBS, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions IHC (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).
Shipping Temperature Blue Ice or 4°C
Specificity Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may be subject to change
Storage Temperature -20°C
Trademark MOLECULAR SIGNATURE
Applications FA, ELISA, IHC, DB, IP, ICC/IF, FCM
Description IHC ; ICC ; IF ; ELISA ; DB ; IP ; FCM
Dilution IHC (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Kim H.W., Murakami A., Williams M.V., and Ohigashi H. (2003) Carcinogenesis 24(2): 235-241.

2. Pilger A. and Rudiger H.W. (2006) Int Arch Occup Environ Health. 80(1): 1-15.

3. Malins D.C. and Haimanot R. (1991) Cancer Res. 51(19): 5430-5432.

4. Kvam E. and Tyrrell R.M. (1997) Carcinogenesis 18(11): 2281-2283.

5. Kowluru R.A., Atasi L., and Ho Y.S. (2006) Invest Ophthalmol Vis Sci 47(4): 1594-9.

6. Bowers R. et al. (2004) Am J Respir Crit Care Med. 169(6): 764-9.

7. Cui J., Holmes E.H., Greene T.G., and Liu P.K. (2000) Faseb J. 14(7): 955-67.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900800-1 SMC-155D Unconjugated 100 ug $306
Add to Cart
BHA11900800-2 SMC-155C Unconjugated 25 ug $144
Add to Cart
BHA11900800-3 SMC-155D-A390 ATTO 390 100 ug $360
Add to Cart
BHA11900800-4 SMC-155D-A488 ATTO 488 100 ug $359
Add to Cart
BHA11900800-5 SMC-155D-A594 ATTO 594 100 ug $359
Add to Cart
BHA11900800-6 SMC-155D-ALP Alkaline Phosphatase 100 ug $352
Add to Cart
BHA11900800-7 SMC-155D-APC APC 100 ug $358
Add to Cart
BHA11900800-8 SMC-155D-BI Biotin 100 ug $354
Add to Cart
BHA11900800-9 SMC-155D-FITC FITC 100 ug $350
Add to Cart
BHA11900800-10 SMC-155D-HRP HRP 100 ug $345
Add to Cart
BHA11900800-11 SMC-155D-PCP PerCP 100 ug $358
Add to Cart
BHA11900800-12 SMC-155D-RPE RPE 100 ug $355
Add to Cart
BHA11900800-13 SMC-155S Unconjugated 12 ug $44
Add to Cart