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Home / Primary Antibodies / Monoclonal Antibodies

DMPO Antibody, Clone N1664A - SMC-189

SKU : BHA11901415

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StressMarq Biosciences Inc.

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Mfr.No.	SMC-189, SMC-189D, SMC-189D-A390, SMC-189D-A488, SMC-189D-A594, SMC-189D-ALP, SMC-189D-APC, SMC-0189D-BI, SMC-0189D-FITC, SMC-189D-HRP, SMC-189D-PCP, SMC-189D-RPE, SMC-189S
Alternative Names	5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide Antibody, DMPO nitrone adduct Antibody, 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-189 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-DMPO Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
Immunogen Region	5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
Isotype	IgG1
Launch Date	2011/01/06
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	DMPO
Clone No	N1664A
Research Area	Cancer, Oxidative Stress, Cell Signaling, Metabolism, Mitochondrial Metabolism, Mitochondrial Markers, Redox Metabolism

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25µg); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
Shipping Temperature	Blue Ice or 4°C
Specificity	Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, ELISA, IHC, IP, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF ; IP ; ELISA ; AM
Dilution	WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25ug); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:14731481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:7375. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251256. 7. Free Radic Biol Med. (2009) April 1; 46(7): 853865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., et al. (2009) Free Radic. Med.and Biol. 46: 454-461.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11901415-1	SMC-189D	Unconjugated	100 ug	$341		Add to Cart
BHA11901415-2	SMC-189D-A390	ATTO 390	100 ug	$395		Add to Cart
BHA11901415-3	SMC-189D-A488	ATTO 488	100 ug	$394		Add to Cart
BHA11901415-4	SMC-189D-A594	ATTO 594	100 ug	$394		Add to Cart
BHA11901415-5	SMC-189D-ALP	Alkaline Phosphatase	100 ug	$387		Add to Cart
BHA11901415-6	SMC-189D-APC	APC	100 ug	$393		Add to Cart
BHA11901415-7	SMC-0189D-BI	Biotin	100 ug	$363		Add to Cart
BHA11901415-8	SMC-0189D-FITC	FITC	100 ug	$363		Add to Cart
BHA11901415-9	SMC-189D-HRP	HRP	100 ug	$381		Add to Cart
BHA11901415-10	SMC-189D-PCP	PerCP	100 ug	$393		Add to Cart
BHA11901415-11	SMC-189D-RPE	RPE	100 ug	$391		Add to Cart
BHA11901415-12	SMC-189S	Unconjugated	12 ug	$44		Add to Cart

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