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Home / Antibodies

Cyclin D1 Antibody / CCND1 (V2830PE)

SKU : BHA17122835

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NSJ Bioreagents

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Mfr.No.	V2830PE-100T
Clonality	Monoclonal (mouse origin)
Description	Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Formulation	500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	Human recombinant full length protein was used as the immunogen for the Cyclin D1 antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the RBP1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P24385

Format	PE Conjugate
Purity	Protein G affinity chromatography
Storage	Store the Cyclin D1 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

Applications	FACS
Description	Flow Cytometry: 5ul per test per one million cells in 100ul

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17122835	V2830PE-100T	100 Tests	$499		Add to Cart

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