Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Formulation
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host
Mouse
Immunogen Region
Human recombinant full length protein was used as the immunogen for the Cyclin D1 antibody.
Isotype
IgG2a,kappa
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Subcellular Location
Nuclear
Uniprot
P24385
Clone No
SPM587
Concentration
0.1 mg/ml
Format
PE Conjugate
Purification
Protein G affinity chromatography
Storage
Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Applications
FACS
Dilution
Flow Cytometry: 5ul per test per one million cells in 100ul