This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A partial recombinant protein (aa 82-238) was used as the immunogen for the CELA3B antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the CELA3B antibody should be determined by the researcher.
Uniprot
P08861
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CELA3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).