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Home / Primary Antibodies / Monoclonal Antibodies

CD74 Antibody, Clone 1B8 - SMC-267

BHA11902386
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+1 866.986.9598
Email
[email protected]
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Swissprot P04233
Accession Number NP_001020329.1
Gene Id 972
Target CD74
Clone No 1B8
Research Area Immunology, Adaptive Immunity, B Cell CD Markers, B Cells
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 1 mg/ml
Dilutions WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment.
Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~33 kDa protein.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications WB, ICC/IF
Description WB ; ICC ; IF
Dilution WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9.

2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9.

3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816.

4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11.

5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165.

6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11902386-1 SMC-267D Unconjugated 100 ug $330
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BHA11902386-2 SMC-267D-A390 ATTO 390 100 ug $384
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BHA11902386-3 SMC-267D-A488 ATTO 488 100 ug $383
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BHA11902386-4 SMC-267D-A594 ATTO 594 100 ug $383
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BHA11902386-5 SMC-267D-ALP Alkaline Phosphatase 100 ug $376
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BHA11902386-6 SMC-267D-APC APC 100 ug $382
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BHA11902386-7 SMC-267D-BI Biotin 100 ug $378
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BHA11902386-8 SMC-267D-FITC FITC 100 ug $374
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BHA11902386-9 SMC-267D-HRP HRP 100 ug $370
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BHA11902386-10 SMC-267D-PCP PerCP 100 ug $382
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BHA11902386-11 SMC-267D-RPE RPE 100 ug $380
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BHA11902386-12 SMC-267S Unconjugated 12 ug $44
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