Rabbit Anti-Human CD34 Polyclonal

Mfr.No.	SPC-1270, SPC-1270D, SPC-1270D-A390, SPC-1270D-A488, SPC-1270D-A594, SPC-1270D-BI, SPC-1270D-FITC
Immunogen Species	Human
Alternative Names	Hematopoietic progenitor cell antigen CD34 Antibody, CD34 Antibody, Cluster designation 34 Antibody, Mucosialin Antibody, HPCA1 Antibody
Antigen	CD34
Categories	Antibodies
Cellular Localization	Membrane
Certificate Of Analysis	A 1:1000 dilution of SPC-1270 was sufficient for detection of CD34 in 10 ug of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human CD34 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A synthesized peptide derived from human CD34, Homo Sapiens (Human)
Immunogen Region	A synthesized peptide derived from human CD34
Isotype	IgG
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human
Tissue Specificity	Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Swissprot	P28906
Accession Number	NP_001764.1
Gene Id	947
Target	CD34
Research Area	Immunology, Cell Type Markers, CD Markers, Stem Cells, Lineage Markers, Mesenchymal Stem Cells, Negative Markers, Hematopoietic Progenitors, HP Surface Molecules, Endothelial Progenitors, Mesoderm

Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Scientific Background	CD34, also known as gp105-120, is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation sites. It mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of total CD34
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB
Description	WB
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Sidney L.E., Branch M.J., Dunphy S.E., Dua H.S., Hopkinson A. (2014). Stem Cells 32(6): 13801389. 2. Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B. (1992). Journal of Immunology. 148 (1): 267271. 3. Nielsen J.S., McNagny K.M. (2008). Journal of Cell Science 121 (Pt 22): 36833692. 4. https://en.wikipedia.org/wiki/CD34

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11917082-1	SPC-1270D	Unconjugated	100 ul	$286	Add to Cart
BHA11917082-2	SPC-1270D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11917082-3	SPC-1270D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11917082-4	SPC-1270D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11917082-5	SPC-1270D-BI	Biotin	100 ul	$334	Add to Cart
BHA11917082-6	SPC-1270D-FITC	FITC	100 ul	$330	Add to Cart

CD34 Antibody - SPC-1270