CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An E. coli-derived mouse protein (amino acids E19-S454) was used as the immunogen for the Caspase 9 antibody.
Isotype
Rabbit IgG
Species Reactivity
Mouse, Rat
Note
Optimal dilution of the Caspase 9 antibody should be determined by the researcher.
Uniprot
Q8C3Q9
Format
Antigen affinity purified
Purity
Affinity purified
Storage
After reconstitution, the Caspase 9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.