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Home / Primary Antibodies / Polyclonal Antibodies

CaMKII Antibody (pThr286) - SPC-931

BHA11911574
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+1 866.986.9598
Email
[email protected]
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Swissprot Q9UQM7
Accession Number NP_741960
Gene Id 815
Target CaMKII (pThr286)
Research Area Neurotransmission, Neurotransmitter Receptors, Cell Signaling, Cardiovascular System, Heart, Hypertrophy, Cancer, Cancer Metabolism, Cancer Carbohydrate Metabolism, Metabolism, Carbohydrate Metabolism, Neuroscience
Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions WB (1:250); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+ auto inhibitory effect and activates the kinase (8). /calmodulin to its regulatory domain releases its This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Shipping Temperature Blue Ice or 4°C
Specificity Detects 54.03 kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications AM, WB
Description WB ; AM
Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013.

2. Barria A. et al. (1997) Science 276: 2042–2045.

3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798.

4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.

5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.

6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.

7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.

8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12.

9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.

10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.

11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.

12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

13. Shifman J. M., Choi M. H., Mihalas S, Mayo S. L., Kennedy M. B. 2006 Proc. Natl. Acad. Sci. U.S.A. 103: 13968-13973.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11911574-1 SPC-931D Unconjugated 100 ul $331
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BHA11911574-2 SPC-931D-A390 ATTO 390 100 ul $385
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BHA11911574-3 SPC-931D-A488 ATTO 488 100 ul $384
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BHA11911574-4 SPC-931D-A594 ATTO 594 100 ul $384
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BHA11911574-5 SPC-931D-BI Biotin 100 ul $380
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BHA11911574-6 SPC-931D-FITC FITC 100 ul $375
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