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Home / Primary Antibodies

Caldesmon / CAD Antibody (HMW) (V7159SAF)

SKU : BHA17126075

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NSJ Bioreagents

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Mfr.No.	V7159SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Caldesmon is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). (UniProt)
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant full-length human protein was used as the immunogen for the CAD antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Titering of the CAD antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Q05682

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the CAD antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17126075	V7159SAF-100UG	100 ug	$499		Add to Cart

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