This recombinant Beta Actin antibody reacts to b-Actin (ACTB/Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
A peptide corresponding to the N-terminus of b-Actin was used as the immunogen for this recombinant Beta Actin antibody.
Isotype
Rabbit IgG
Species Reactivity
All Species
Note
The stated application concentrations are suggested starting points. Titration of the recombinant Beta Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Uniprot
P60709
Format
Biotin Conjugate
Purity
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant Beta Actin antibody at -20oC.
Applications
WB, IP, ICC, IHC, ChIP
Description
Western Blot: 1:1000,Immunoprecipitation: 1:200,Chromatin IP (ChIP): 1:200,Immunocytochemistry: 1:200,Immunohistochemistry: 1:200 (1)