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Home / Primary Antibodies

Bcl-2 Antibody (V3543)

SKU : BHA17114176

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NSJ Bioreagents

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Mfr.No.	V3543, V3543-20UG, V3543-100UG
Antigen	Bcl-2
Clonality	Monoclonal
Description	This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	Recombinant full-length human protein was used as the immunogen for the recombinant Bcl-2 antibody.
Isotype	IgG1,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	Yes
Subcellular Location	Cytoplasmic, membrane

Uniprot	P10415
Clone No	rBCL2/796

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the recombinant Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	FACS, IF, WB, IHC-P
Dilution	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17114176-1	V3543-20UG	20ug	$219		Add to Cart
BHA17114176-2	V3543-100UG	100ug	$499		Add to Cart

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