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Home / Primary Antibodies / IHC Antibodies

ATR (Ab-428) Antibody

SKU : BHA10557501

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA267100
Alternative Names	FRP1; MEC1; SCKL; SCKL1;
Categories	Primary Antibodies
Clonality	polyclonal
Description	ATR encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.Zhou, X.Z. et al. (1999) Cell Mol. Life Sci. 56, 788-806.Pinna, L.A. and Ruzzene, M. (1996) Biochim. Biophys. Acta 1314, 191-225.Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-908.
Host	Rabbit
Immunogen	Peptide sequence around aa.426~430 (G-I-S-P-K) derived from Human ATR.
Involvement In Disease	Seckel syndrome 1 (SCKL1); Cutaneous telangiectasia and cancer syndrome, familial (FCTCS)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	ATR encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. Zhou, X.Z. et al. (1999) Cell Mol. Life Sci. 56, 788-806. Pinna, L.A. and Ruzzene, M. (1996) Biochim. Biophys. Acta 1314, 191-225. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186. Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-908.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total ATR protein.
Subcellular Location	Nucleus, Nucleus, PML body, Chromosome

Uniprot	Q13535
Gene Id	545
Database Links	HGNC:882 OMIM:210600 KEGG:hsa:545 STRING:9606.ENSP00000343741 UniGene:Hs.271791
Function	Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
Pathway	p53 signaling pathway Cell cycle Cellular senescence
Protein Families	PI3/PI4-kinase family, ATM subfamily
Tissue Specificity	Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Weight	297,479 Da

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, IHC

Description

ELISA ; IHC, IHC ; ELISA

Dilution

IHC	1:50-1:100

Tested Application

ELISA,IHC;IHC:1:50-1:100

Datasheet
ELISA Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557501	CSB-PA267100	100ul	$297		Add to Cart

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