Recombinant anti-TFR (Transferrin Receptor), which recognizes TFR. The purified antibody was enzymatically biotinylated using Avi-tagTM technology. This affinity purified antibody has been tested for specific binding to purified human TFR1 (BPS Bioscience #101287) in an ELISA binding assay.
assay conditions: An ELISA binding assay was performed with this antibody and its target protein, TFR1 (BPS Bioscience #101287). The target protein was coated onto an assay plate overnight at 2 ug/ml at 4°. The next day, the plate was washed on blocked. Then, serial dilutions of the antibody were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Then we measured absorbance on a plate reader at 450 nm. EC50 = 0.61 nM
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host
HEK293
Instructions for use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Molecular Weight
Heavy Chain: 50 kDa; Light Chain: 24 kDa + glycans
Species
Human
Synonyms
Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, Transferrin receptor protein 1, serum form (sTfR), CD71, TFRC