This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Host
Rabbit
Immunogen
The antibody was produced against synthesized phosphopeptide derived from human MDM2 around the phosphorylation site of Serine 166 (A-I-SP-E-T).
Isotype
IgG
Quantity
100 µg
Reactivity
Human
Recombinant
TRUE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
100µg/100ul
Description
Specificity: MDM2 (phospho-Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Serine 166.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP, IHC
Description
WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:200, WB ; IP ; IHC