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Home / Primary Antibodies / Monoclonal Antibodies

Anti-Claudin-18 Isoform 2 IgG Antibody

SKU : BHA18200078

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BPS Bioscience

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Mfr.No.	101564-1
Description	Anti-Claudin-18 isoform 2 (CLDN18.2) IgG1 antibody is a purified recombinant antibody which recognizes human CLDN18.2 antigen. This antibody has been tested for specific binding affinity to purified human CLDN18.2 protein. assay conditions: Functional validation: The antibody was validated by measuring its binding to CLDN18.2 antigen in ELISA. The CLDN18.2 protein was coated onto a 96-well plate overnight at 4°C (50 ul/well at a concentration of 2 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728 with DDM/CHS detergent (dodecyl-beta-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified anti-CLDN18.2 antibody (BPS Bioscience #101564), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with Colorimetric HRP substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host	HEK293
Molecular Weight	Total: 150 kDa; Light Chain: 24 kDa; Heavy Chain: 51 kDa
Species	Human
Synonyms	CLDN18, UNQ778, PRO1572, Claudin 18.2
Note	Avoid freeze/thaw cycles.

Format	Aqueous buffer solution
Purification	Protein A affinity
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200078	101564-1	50 ug	$390.50		Add to Cart

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