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Home / Primary Antibodies / Monoclonal Antibodies

Anti-CD19-Anti-CD3 IgG format Bispecific Antibody

SKU : BHA18200060

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BPS Bioscience

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Mfr.No.	101076-1
Clonality	Monoclonal (Bispecific)
Description	Anti-CD19-Anti-CD3 IgG format bispecific antibody is a purified recombinant human bispecific antibody with T cell Engager. This bispecific antibody has been tested for specific activity in the functional reporter assay using NFAT-luc reporter Jurkat cell line (BPS Bioscience #60621) in the presence of CD19-CHO cells (BPS Bioscience #79561-H) that overexpress CD19. assay conditions: Experimental design and assay protocol for measuring anti CD19-anti CD3 functional activity using NFAT-luc reporter Jurkat cell line:Jurkat effector cells with endogenous TCR/CD3 and transfected reporter NFAT-luc (BPS Bioscience #60621) are incubated with increasing concentrations of anti-CD19 x anti-CD3 bispecific antibody in the presence of CD19-CHO cells (BPS Bioscience #79561-H) or CHO cells (ATCC #CCL-61). 1. Seed CHO and CD19-CHO cells at 30,000 cells/well and allow a few hours for the cells to attach in a 96-well clear bottom white plate. 2. Seed Jurkat cells at 30,000 cells/well. 3. Add the bispecific antibody at a recommended dilution range of 100 fM-100 nM. The bispecific antibody simultaneously binds to TCR/CD3 on the NFAT-luc Jurkat reporter cells and tumor antigen CD19 on CD19-CHO cells. 4. After 16 hours the luciferase activity is measured using ONE-Step luciferase assay (BPS Bioscience #60690) per recommended protocol. The bispecific antibody interaction stimulates NFAT-luciferase activity.
Formulation	8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Molecular Weight	Heavy Chain: 50 kDa; Light Chain: 24 kDa
Species	Human
Note	Avoid freeze/thaw cycles.

Format	Aqueous buffer solution
Purification	Protein A affinity purification of the IgG-tag protein from HEK293 cells.
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200060	101076-1	50 ug	$429		Add to Cart

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