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Home / Primary Antibodies / Monoclonal Antibodies

Anti-CD123 IgG Antibody, Biotin-Labeled

SKU : BHA18200065

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BPS Bioscience

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Mfr.No.	101140-2
Description	Biotinylated anti-CD123 IgG antibody is a purified recombinant antibody that recognizes human CD123 antigen. This antibody has been tested for specific binding affinity to purified human CD123 protein. assay conditions: Functional validation: The antibody was validated by measuring anti-CD123 binding to CD123 antigen in ELISA assay. The CD123 protein (BPS Bioscience #101035) was coated onto a 96-well plate overnight at 4°C (50 ul/well at a concentration of 4 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified biotinylated anti-CD123 antibody (BPS Bioscience #101140), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host	Heavy chain (HC) and Light chain (LC) co-expressed in HEK293
Isotype	IgG1
Molecular Weight	Total: 150 kDa; HC: 51 kDa; LC: 24 kDa
Species	Human
Synonyms	Cell division cycle protein 123 homolog, Protein D123, HT-1080, PZ32, CDC123, C10orf7, D123
Note	Avoid freeze/thaw cycles.

Uniprot	O75794

Format	Aqueous buffer solution
Purification	Protein A affinity
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200065	101140-2	100 ug	$522.50		Add to Cart

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