Rabbit Anti-Human AKT1 (pTyr315) Polyclonal

Mfr.No.	SPC-911, SPC-911D, SPC-911D-A390, SPC-911D-A488, SPC-911D-A594, SPC-911D-BI, SPC-911D-FITC
Immunogen Species	Human
Alternative Names	AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody
Antigen	AKT1
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus, Cell Membrane
Certificate Of Analysis	A 1:250 dilution of SPC-911 was sufficient for detection of AKT1 (pTyr315) in 10 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human AKT1 (pTyr315) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A phospho-specific peptide corresponding to residues surrounding Tyr315 of human Akt1 (AA312-318), Homo Sapiens (Human)
Immunogen Region	A phospho-specific peptide corresponding to residues surrounding Tyr315 of human Akt1 (AA312-318)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human
Tissue Specificity	Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.

Swissprot	P31749
Accession Number	NP_005154
Gene Id	207
Target	AKT1 (pTyr315)
Research Area	Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKB / AKT, Cancer, Apoptosis, Metabolism, Metabolism processes

Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions	WB (1:250); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Scientific Background	AKT1 is a protein-serine/threonine kinase which is inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, AKT1 is recruited to the cell membrane and phosphorylated to become active. This leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. AKT1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and activatory phosphorylation of mTOR. Upregulation of the PI3K/AKT pathways has been implicated in a variety of cancers.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 55.686 kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, WB
Description	WB ; AM
Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11911214-1	SPC-911D	Unconjugated	100 ul	$331	Add to Cart
BHA11911214-2	SPC-911D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11911214-3	SPC-911D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11911214-4	SPC-911D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11911214-5	SPC-911D-BI	Biotin	100 ul	$380	Add to Cart
BHA11911214-6	SPC-911D-FITC	FITC	100 ul	$375	Add to Cart

AKT1 Antibody (pTyr315) - SPC-911

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