This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant protein was used as the immunogen for the Muscle Actin antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the Muscle Actin antibody should be determined by the researcher.
Uniprot
P62736, P68032 & P68133
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the Muscle Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Applications
WB, IHC-P
Description
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-2ug/ml