Acetyl-Histone H2A.X (Lys9) Antibody

Mfr.No.	AF3823
Clonality	Polyclonal
Host	Rabbit
Immunogen	A synthesized peptide derived from human Histone H2A.X around the acetylation site of Lys9., A synthesized peptide derived from human Histone H2A.X around the acetylation site of Lys9.
Isotype	IgG
Reactivity	Human, Mouse, Rat

Rgd	AB_2847137
Uniprot	P16104
Gene Id	3014
Gene Name	H2AFX

Storage	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IHC, ELISA
Dilution	IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Product Manual	Data Sheet

AF3823 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3823 at 1/100 staining Human colorectal cancer by IHC-P.

AF3823 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3823 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

AF3823 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3823 at 1/100 staining Human kidney cancer by IHC-P.

AF3823 at 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3823 at 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P.

AF3823 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3823 at 1/100 staining Human ovarian cancer by IHC-P.