Rabbit Anti-Human Abl1 (pTyr257) Polyclonal

Mfr.No.	SPC-903, SPC-903D, SPC-903D-A390, SPC-903D-A488, SPC-903D-A594, SPC-903D-BI, SPC-903D-FITC
Immunogen Species	Human
Alternative Names	Abelson tyrosine protein kinase 1 Antibody, ABL Antibody, c ABL Antibody, p150 Antibody, bcr/abl Antibody
Categories	Antibodies
Cellular Localization	Cytoskeleton, Cytoplasm, Nucleus, Mitochondrion
Certificate Of Analysis	A 1:250 dilution of SPC-903 was sufficient for detection of Abl1 (pTyr257) in 10 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human Abl1 (pTyr257) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A phospho-specific peptide corresponding to residues surrounding Tyr257 of human Abl1 (AA254-260), Homo Sapiens (Human)
Immunogen Region	A phospho-specific peptide corresponding to residues surrounding Tyr257 of human Abl1 (AA254-260)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human
Tissue Specificity	Widely expressed

Swissprot	P00519
Accession Number	NP_005148
Gene Id	25
Target	Abl1 (pTyr257)
Research Area	Cell Signaling, Protein Phosphorylation, Tyrosine Kinases, Epigenetics and Nuclear Signaling, Transcription, Cancer, Tumor Biomarkers, Oncoproteins

Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions	WB (1:250); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Widely expressed
Scientific Background	ABL1 is a widely-expressed, non-receptor protein-tyrosine kinase. It is stabilized in the inactive form by an association between the SH3 domain and the SH2-TK linker region, interactions of the N-terminal cap, and contributions from an N-terminal myristoyl group and phospholipids. It is activated by autophosphorylation as well as by Src-family kinase-mediated phosphorylation. It can also be activated by RIN1 binding to the SH2 and SH3 domains. As it is stimulated by cell death inducers and DNA-damage, it is likely that the wild-type form of Abl1 is actually a tumour -suppressor protein. The normal active form of Abl1 is nuclear, and it is sequestered into the cytoplasm by interaction with 14-3-3 through Thr-735. It localizes to the mitochondria under conditions of oxidative stress.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 122.945 kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, WB
Description	WB ; AM
Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11911070-1	SPC-903D	Unconjugated	100 ul	$331	Add to Cart
BHA11911070-2	SPC-903D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11911070-3	SPC-903D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11911070-4	SPC-903D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11911070-5	SPC-903D-BI	Biotin	100 ul	$380	Add to Cart
BHA11911070-6	SPC-903D-FITC	FITC	100 ul	$375	Add to Cart

Abl1 Antibody (pTyr257) - SPC-903