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Home / Primary Antibodies / Monoclonal Antibodies

6*His, His-Tag antibody

SKU : BHA10800008

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Mfr.No.	FNab00008
Alternative Names	6His ; 6His ; HisTag ; 6histag ; 6XHis ; HHHHHH ; HIS ; HisTag ; HISTIDINETAGGED
Background	Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Categories	Primary Antibodies ; Tag & Cell Markers
Clonality	monoclonal
Description	Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Host	Mouse
Isotype	IgG1
Reactivity	Recombinant Protein
Regulatory	RUO
Synonyms	6His, 6His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag, HISTIDINE TAGGED

Clone No	1C4

Form	liquid
Format	liquid
Purification	Protein A+G purification
Purity	>=95% as determined by SDS-PAGE
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)

Applications

ELISA, WB, IP, IF

Description

ELISA ; WB ; IP ; IF

Dilution

WB	1:1000-1:10000
IP	1:1000-1:10000
IF	1:20-1:100

Tested Application

WB: 1:1000-1:10000; IP: 1:1000-1:10000; IF: 1:20-1:100

Manual	FNab00008.pdf

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SKU	Supplier No.	Size	Your price	Quantity
BHA10800008	FNab00008	100ug	$260		Add to Cart

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