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Home / Primary Antibodies / Monoclonal Antibodies

4-Hydroxy-2-hexenal Antibody, Clone 12F4 - SMC-537

SKU : BHA11905863

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StressMarq Biosciences Inc.

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Mfr.No.	SMC-537, SMC-537D, SMC-537D-A390, SMC-537D-A488, SMC-537D-A594, SMC-537D-ALP, SMC-537D-APC, SMC-537D-BI, SMC-537D-FITC, SMC-537D-HRP, SMC-537D-PCP, SMC-537D-RPE, SMC-537S
Alternative Names	4-Hydroxy-2-hexenal (4-HHE) Antibody, 4-Hydroxy-2-hexenal Antibody, 4-HHE Antibody, MG Antibody, MG-modified protein Antibody, 4-Hydroxy-2-hexenal-modified , 4-Hydroxy-2-hexenal Antibody, 4-hydroxy Hexenal Antibody, HHE Antibody, 4-HHE Antibody
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-537 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 ug of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-4-Hydroxy-2-hexenal Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
Immunogen Region	Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
Isotype	IgG1
Launch Date	2017/10/10
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	4-Hydroxy-2-hexenal
Clone No	12F4
Research Area	Cancer, Oxidative Stress, Lipid peroxidation

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C
Trademark	MOLECULAR SIGNATURE

Applications	ELISA, WB
Description	WB ; ELISA
Dilution	WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11905863-1	SMC-537D	Unconjugated	100 ug	$409		Add to Cart
BHA11905863-2	SMC-537D-A390	ATTO 390	100 ug	$463		Add to Cart
BHA11905863-3	SMC-537D-A488	ATTO 488	100 ug	$462		Add to Cart
BHA11905863-4	SMC-537D-A594	ATTO 594	100 ug	$462		Add to Cart
BHA11905863-5	SMC-537D-ALP	Alkaline Phosphatase	100 ug	$455		Add to Cart
BHA11905863-6	SMC-537D-APC	APC	100 ug	$461		Add to Cart
BHA11905863-7	SMC-537D-BI	Biotin	100 ug	$458		Add to Cart
BHA11905863-8	SMC-537D-FITC	FITC	100 ug	$453		Add to Cart
BHA11905863-9	SMC-537D-HRP	HRP	100 ug	$449		Add to Cart
BHA11905863-10	SMC-537D-PCP	PerCP	100 ug	$461		Add to Cart
BHA11905863-11	SMC-537D-RPE	RPE	100 ug	$459		Add to Cart
BHA11905863-12	SMC-537S	Unconjugated	12 ug	$44		Add to Cart

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