DNA nucleotidylexotransferase ; DNTT ; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase ; TDT ; Terminal addition enzyme ; Terminal deoxyribonucleotidyltransferase ; Terminal transferase
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
10q24.1
Clonality
monoclonal
Description
Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3'-hydroxyl end of DNA primers without template direction. The enzyme thus provides a unique method for the labeling of the 3' termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. TdT is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.
Host
Mouse
Immunogen
Recombinant human DNTT protein fragment (around aa 85-215) (exact sequence is proprietary)
Isotype
IgG1 Kappa
Positive Control
Jurkat Cells. Thymus
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P04053
Gene Id
1791
Clone No
TdT, 1392
Symbol
DNTT
Weight
58kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
ELISA
Description
Order Ab without BSA, Optimal dilution for a specific application should be determined., ELISA
Dilution
ELISA
Use Ab at 2-4μg/ml for coating
Reviews of Anti-undefined Antibody TdT,1392 Unconjugated BHA10208290