Anti-undefined Antibody GFR,2341 Unconjugated BHA10208201

Mfr.No.	1956-MSM11-P1ABX
Alternative Names	Erbb1 ; ERBB1 ; Errp ; HER1 ; mENA ; PIG61 ; Proto-oncogene c-ErbB-1 ; Receptor Tyrosine Protein Kinase ; ErbB1 ; Urogastrone ; wa2 ; Wa5
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	7p11.2
Clonality	monoclonal
Description	This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Host	Mouse
Immunogen	Recombinant fragment of human EGFR protein (around aa 344-492) (exact sequence is proprietary)
Isotype	IgG2b Kappa
Positive Control	A431 cells. Placenta, Breast, Colon or Bladder cancer
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P00533
Gene Id	1956
Clone No	GFR, 2341
Symbol	EGFR
Weight	~170kDa (wild type) and ~145kDa (vIII variant)

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IHC-F

Dilution

Immunohistology (Formalin-fixed)

1-2μg/ml for 30 minutes at RT